Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Full length protein from human plasma
Molecular Weight	31 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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ox-LDL: LDL(Copper oxidized); Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein.

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Blank control: A431. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (orb10973) Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3 µg/Test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (orb10973), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (orb10973) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence

Filter by Applications

ELISA (1)
IF (2)
IHC (3)

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Chinese Hamster (1)
Human (5)

Stephan Zeibiga, Manuela Büttcherb Silvia Goebela, Jessica Paulia, Annique Hungera, Martin Ungerera, Meinrad Gawazb, Götz Müncha The Scavenger Receptor CD68 Regulates Platelet Mediated Oxidized Low-Density Lipoprotein (oxLDL) eposition in Atherosclerotic Vessels at an Early Stage of Atherosclerosis in LDLR-/-/ApoBec-/- Mice Cell Physiol Biochem, 52, 681-695 (2019)

PubMed: 30921507

Applications

ELISA

Reactivity

Chinese Hamster

Wern Yew Ding, Majd B Protty, Ian G Davies, Gregory Y H Lip Relationship between lipoproteins, thrombosis, and atrial fibrillation Cardiovasc Res, 118, 716–731 (2022)

PubMed: 33483737

Applications

Reactivity

Human

Wei Jiang 1, Chun Gan 1, Xindi Zhou 1, Qing Yang 1, Dan Chen 1, Han Xiao 1, Lujun Dai 2, Yaxi Chen 3, Mo Wang 1, Haiping Yang 4, Qiu Li 5 Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease Cardiovasc Diabetol, 22, 293 (2023)

PubMed: 37891556

Applications

Reactivity

Human

David Freiholtz 1 2, Claudia Reyes-Goya 1 2, Karin Lång 1 2, Otto Bergman 1 2, Christian Olsson 3 4, Malin Granbom Koski 3 4, Michael Dismorr 3 4, Cecilia Österholm 5, Kenneth Caidahl 6 7, Anders Franco-Cereceda 3 4, Per Eriksson 1 2, Anton Gisterå 8, Hanna M Björck Tricuspid Aortic Valve Regurgitation Associate With Ascending Aortic Aneurysm Through Endothelial Activation and Lipoprotein Infiltration Arterioscler Thromb Vasc Biol, (2025)

PubMed: 40740135

Applications

IHC

Reactivity

Human

Uchida, Yasumi et al. Pericoronary Adipose Tissue as Storage and Supply Site for Oxidized Low-Density Lipoprotein in Human Coronary Plaques PLoS One, 11, e0150862 (2016)

PubMed: 27010927

Applications

IHC

Reactivity

Human

Uchida, Yasumi et al. Human pericoronary adipose tissue as storage and possible supply site for oxidized low-density lipoprotein and high-density lipoprotein in coronary artery J Cardiol, 69, 236-244 (2017)

PubMed: 27209423

Applications

IHC

Reactivity

Human