P38 MAPK/ MAPK14 Rabbit Polyclonal Antibody

SKU: orb500604

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Description

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Canine, Equine, Gallus, Human, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human P38 MAPK/ MAPK14 (121-220/360aa)
Target	MAPK14
Molecular Weight	41 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CSAID Binding Protein 1; CSAID binding protein; CSAID-binding protein; Csaids binding protein; CSBP 1; CSBP 2; CSBP; CSBP1; CSBP2; CSPB 1; CSPB1; Cytokine suppressive anti inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; EXIP; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX interacting protein 2; MAX-interacting protein 2; Mitogen Activated Protein Kinase 14; Mitogen activated protein kinase p38 alpha; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MK14_HUMAN; Mxi 2; Mxi2; p38 ALPHA; p38; p38 MAP kinase; p38 MAPK; p38/MAPK; p38 mitogen activated protein kinase; p38ALPHA; p38alpha Exip; PRKM14; PRKM15; RK; SAPK 2A; SAPK2A; Stress Activated Protein Kinase 2A.

Phospho-p38 MAPK (Thr180 + Tyr182) Rabbit Polyclonal Antibody [orb6578]

Applications:	ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Canine, Equine, Gallus, Human, Porcine, Rabbit
Reactivity:	Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 8
Phospho-P38 MAPK (Thr180 + Tyr182) Rabbit Polyclonal Antibody [orb11208]
Applications:
IF, IHC-Fr, IHC-P
Predicted Reactivity:
Canine, Rabbit
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl
Item 1 of 5
Phospho-P38 MAPK (Thr180) Rabbit Polyclonal Antibody [orb7089]
Applications:
FC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:
Canine, Equine, Gallus, Porcine, Rabbit
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl
Item 1 of 4
Phospho-p38 MAPK (Tyr323) Rabbit Polyclonal Antibody [orb7090]
Applications:
IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:
Canine, Equine, Gallus, Human, Porcine, Rabbit, Rat
Reactivity:
Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl, 50 μl, 200 μl

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P38 MAPK/ MAPK14 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (P38 MAPK/MAPK14) Polyclonal Antibody, Unconjugated (orb500604) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-MAPK14 (orb500604) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Muscle tissue lysates, Lane 3: Rat Muscle tissue lysates, Primary: Anti-P38 MAPK/ MAPK14 (orb500604) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 46 kDa.

Quick Database Links

Gene Symbol

MAPK14

UniProt

Q16539

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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