P53 Recombinant Rabbit Monoclonal Antibody

SKU: orb1816720

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Description

Research Area

Cancer Research, Tumor Suppressors, p53 Signaling Pathway

Images & Validation

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Item 1 of 3

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:2000-20000, IHC-P=1:200-1000, IF=1:200-1000, IHC-F=1:200-1000, Flow-Cyt=1ug/Test, ICC/IF=1:100-500
Reactivity	Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	3H6
Immunogen	A synthesized peptide derived from human p53 (360-393aa)
Target	TP53
Molecular Weight	43 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, Glycerol, rAlbumin.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

P53 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (p53) Monoclonal Antibody, Unconjugated (orb1816720) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (p53) Monoclonal Antibody, Unconjugated (orb1816720) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (p53) Monoclonal Antibody, Unconjugated (orb1816720) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

TP53

UniProt

P04637

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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