PCGF4/BMI1 Rabbit Polyclonal Antibody (FITC)

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-PCGF4 BMI1 antibody (orb7539), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control (blue line): Mouse kidney (Black). Primary Antibody (green line): Rabbit Anti-PCGF4 BMI1/FITC antibody (orb7539), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (5 min at -20°C) and then permeabilized with ice-cold mehtanol for 20 min at -20°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-PCGF4/BMI1 Antibody, conjugated (orb7539) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.