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Phospho-14-3-3 epsilon (Thr232) Rabbit Polyclonal Antibody

SKU: orb6361

Description

Phospho-14-3-3 epsilon (Thr232) Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cytoplasmic, Neurology, Signal Transduction, Synapse Markers

Images & Validation

Tested ApplicationsFC
Dilution rangeFlow-Cyt=1μg/Test
ReactivityHuman
Predicted ReactivityBovine, Mouse, Rat, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human 14-3-3E around the phosphorylation site of Thr232 LW(p-T)SD
TargetYWHAE
Molecular Weight29 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

YWHAE(phospho T232); 14-3-3 epsilon(phospho Thr232); 14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 E; 14-3-3 protein epsilon; 14-3-3E; 14-3-3ε; 1433E_HUMAN; KCIP 1; KCIP-1; KCIP1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; Protein kinase C inhibitor protein1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5-monooxygenase activation protein epsilon polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide; YWHAE.

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Phospho-14-3-3 epsilon (Thr232) Rabbit Polyclonal Antibody

Blank control (blue line): MCF7, fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice, Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 epsilon (Thr232) antibody (orb6361), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

UniProt Details

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Protocol Information

FC
Flow Cytometry
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Phospho-14-3-3 epsilon (Thr232) Rabbit Polyclonal Antibody (orb6361)

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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