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Phospho-AMPK alpha 1 (Thr183) + AMPK alpha 2 (Thr172) Rabbit Polyclonal Antibody
SKU: orb182418
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 1 around the phosphorylation site of Thr183 LR(p-T)SC |
| Target | PRKAA1 |
| Molecular Weight | 63 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−AMPK alpha 1(phospho T183)/AMPK alpha 2(phospho T172); AMPK alpha 1(phospho T183)+AMPK alpha 2(phospho T172); AMPK alpha 1(phospho T183); phospho-AMPK alpha 1(T183); phospho-AMPK alpha 1(Thr183); phospho-AMPK alpha 2(T172); phospho-AMPK alpha 2(Thr172); 5''-AMP-activated protein kinase catalytic subunit alpha-1; AAPK1_HUMAN; AAPK2_HUMAN; ACACA kinase; Acetyl-CoA carboxylase kinase; AMPK alpha 1 chain; AMPK alpha 2 chain; AMPK; AMPK subunit alpha-1; AMPK1; AMPK2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; PRKAA; PRKAA1; PRKAA2; Protein kinase AMP activated alpha 1 catalytic subunit; Protein kinase AMP activated alpha 2 catalytic subunit; Tau-protein kinase PRKAA1; AMPKα1; AMPK α1; AMPKα 1; AMPK α 1; AMPK AMPK-α1; AMPK-α-1; α-1.
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Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (orb182418) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (orb182418) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (orb182418) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Quick Database Links
Gene Symbol
PRKAA1
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Datasheet
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Phospho-AMPK alpha 1 (Thr183) + AMPK alpha 2 (Thr172) Rabbit Polyclonal Antibody (orb182418)
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