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Phospho-EP300 (Ser1834) Rabbit Polyclonal Antibody
SKU: orb6262
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rat, Zebrafish |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human EP300 around the phosphorylation site of Ser1834 MA(p-S)MQ |
| Target | EP300 |
| Molecular Weight | 266 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−EP300_HUMAN; Histone acetyltransferase p300; EC:2.3.1.48; p300 HAT; E1A-associated protein p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; Protein 2-hydroxyisobutyryltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300; P300; KAT3B; MKHK2; RSTS2;
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Ep300 (Ser1834)) Polyclonal Antibody, Unconjugated (orb6262) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Ep300 (Ser1834) (orb6262) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 266 kD, Observed band size: 266 kD.
Quick Database Links
Gene Symbol
EP300
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Phospho-EP300 (Ser1834) Rabbit Polyclonal Antibody (orb6262)
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