Phospho-HEF1 (Ser369) Rabbit Polyclonal Antibody

SKU: orb184057

Description

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Bovine, Equine, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide derived from human HEF1 around the phosphorylation site of Ser369 RL(p-S)FS
Target	NEDD9
Molecular Weight	93 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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HEF1(phospho S369); p-HEF1(phospho S369); Cas like docking; Cas scaffolding protein family member 2; CAS-L; CAS2; CasL; CASL_HUMAN; CASS2; Crk associated substrate related; Crk associated substrate related protein; CRK-associated substrate-related protein; dJ49G10.2(Enhancer of Filamentation 1(HEF1)); dJ49G10.2; dJ761I2.1(enhancer of filamentation(HEF1)); dJ761I2.1; Enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF 1; HEF1

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Phospho-HEF1 (Ser369) Rabbit Polyclonal Antibody

Sample: Lane 1: Human A549 cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human HepG2 cell lysates, Primary: Anti-phospho-HEF1 (Ser369) (orb184057) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 93 kDa, Observed band size: 105, 115 kDa.

Quick Database Links

Gene Symbol

NEDD9

UniProt

Q14511

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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