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Phospho-IKK beta (Ser740) Rabbit Polyclonal Antibody

SKU: orb5522

Description

Phospho-IKK beta (Ser740) Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsICC
Dilution rangeICC/IF=1:50-200
ReactivityHuman
Predicted ReactivityGallus, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human IKK beta around the phosphorylation site of Ser740 DW(p-S)WL
TargetIKBKB
Molecular Weight87 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

IKK beta(phospho S740); IKK beta(phospho Ser740); p-IKK beta(Ser740); IKKI kappa B kinase 2; IKKβ; IKK β; I kappa B kinase beta; IkBKB; IKK 2; IKK B; IKK2; IKKB; Inhibitor of kappa light chain gene enhancer in B cells; Inhibitor of kappa light polypeptide gene enhancer in B cells; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta; Inhibitor of nuclear factor kappa B kinase beta subunit; Inhibitor of nuclear factor kappa B kinase subunit beta; MGC131801; NFKBIKB; Nuclear factor NF kappa B inhibitor kinase beta; Nuclear factor of kappa light chain gene enhancer in B cells inhibitor; IKKB_HUMAN.

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Phospho-IKK beta (Ser740) Rabbit Polyclonal Antibody

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-IKK beta (Ser740)) polyclonal Antibody, Unconjugated (orb5522) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

UniProt Details

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Protocol Information

ICC
Immunocytochemistry
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Phospho-IKK beta (Ser740) Rabbit Polyclonal Antibody (orb5522)

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
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