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Description
Research Area
Cell Biology
Images & Validation
−
Item 1 of 9
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Canine, Equine, Porcine, Rabbit, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human mTOR around the phosphorylation site of Ser2481 IH(p-S)FI |
| Target | MTOR |
| Molecular Weight | 289 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; 2610315D21Rik; flat; MTOR_HUMAN; MTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin (mTOR); Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; Tyrosine-protein kinase mTOR; 2.7.11.1; MTOR_MOUSE; Rapamycin target protein 1 (RAPT1); MTOR_RAT; mechanistic target of rapamycin kinase; FK506 binding protein 12-rapamycin associated protein 1; mechanistic target of rapamycin (serine/threonine kinase); FK506 binding protein 12-rapamycin associated protein 2; rapamycin target protein; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; rapamycin associated protein FRAP2; dJ576K7.1 (FK506 binding protein 12-rapamycin associated protein 1); mammalian target of rapamycin; mTOR (phospho-S2481); p-mTOR; phospho-mTOR; mTOR (phospho-Ser2481)
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Quick Database Links
Gene Symbol
MTOR
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Filter by Applications
Filter by Species
Seyed Soheil Saeedi Saravi, Alireza Arefidoust, Seyed Sobhan Saeedi Saravi, Rahele Yaftian, Mahdi Bayati, Milad Salehi, Ahmad Reza Dehpour Mammalian target of rapamycin (mTOR)/nitric oxide system possibly modulate antidepressant-like effect of 17α-ethinyl estradiol in ovariectomized mice Biomedicine & Pharmacotherapy, 89, 519 (2017)
Applications
WB
Reactivity
Mouse
Available Sizes
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