Phospho-PAK1 (Thr212) Rabbit Polyclonal Antibody

SKU: orb6638

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Description

Research Area

Cancer Research, Cell Biology, Kinases, Serine/Threonine Kinases

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Canine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human PAK1 around the phosphorylation site of Thr212 PV(p-T)PT
Target	PAK1
Molecular Weight	60 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PAK1(phospho T212); p-PAK1(phospho T212); PAK1(phospho-Thr212); PAK1(phospho T212); alpha PAK; alpha-PAK; MGC130000; MGC130001; p21 activated kinase 1; p21 protein(Cdc42/Rac) activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1 activated kinase 1(yeast Ste20 related); p21/Cdc42/Rac1 activated kinase 1; p21/Cdc42/Rac1-activated kinase 1(STE20 homolog, yeast); p65 PAK; p65-PAK; PAK alpha; PAK-1; Pak1; PAK1_HUMAN; Paka; PAKalpha; Protein kinase MUK2; Rac/p21-activated kinase; Serine/threonine-protein kinase PAK 1; STE20 homolog(yeast); STE20 homolog yeast.

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Phospho-PAK1 (Thr212) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PAK1 (Thr212)) Polyclonal Antibody, Unconjugated (orb6638) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PAK1 (Thr212)) Polyclonal Antibody, Unconjugated (orb6638) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PAK1 (Thr212)) Polyclonal Antibody, Unconjugated (orb6638) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PAK1

UniProt

Q13153

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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