Phospho-PIK3C3 (Ser282) Rabbit Polyclonal Antibody

SKU: orb6703

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Description

Research Area

Cell Biology, Lipid Metabolism, Lipid Signaling, Signal Transduction, TLR Signaling Pathway

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human PIK3C3 around the phosphorylation site of Ser282 GP(p-S)DH
Target	PIK3C3
Molecular Weight	98 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PIK3C3(phospho S282); p-PIK3C3(phospho S282); p-PIK3C3(Ser282); PI 3 Kinase Class 3; Phosphatidylinositol 3 kinase catalytic subunit type 3; Phosphatidylinositol 3 kinase class 3; Phosphatidylinositol 3 kinase p100 subunit; phosphoinositide 3 kinase class 3; PI3 kinase type 3; PI3K type 3; PIK3C3; PtdIns 3 kinase type 3; Vps 34; Vps34; hVps34; MGC61518; PK3C3_HUMAN.

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Phospho-PIK3C3 (Ser282) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PIK3C3 (Ser282)) Polyclonal Antibody, Unconjugated (orb6703) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PIK3C3 (Ser282)) Polyclonal Antibody, Unconjugated (orb6703) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PIK3C3

UniProt

Q8NEB9

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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