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Phospho-PKC Epsilon (Ser729) Rabbit Polyclonal Antibody
SKU: orb158163
Description
Research Area
Serine/Threonine Kinase Pathway, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution Range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Rat, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human PKCE around the phosphorylation site of Ser729 FS(p-Y)FG |
| Target | PRKCE |
| Molecular Weight | 84 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PKC epsilon(phospho S729); PKC Epsilon(phospho Ser729); p-PKC Epsilon(Ser729); PKC-A; PKC alpha; AAG6; Aging associated gene 6; PICK 1; PKC A; PKCA; PKC A; PRKCA; Protein Kinase C alpha; Protein kinase C alpha type; KPCE_HUMAN.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-PKC Epsilon (Ser729)) Polyclonal Antibody, Unconjugated (orb158163) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Quick Database Links
Gene Symbol
PRKCE
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Phospho-PKC Epsilon (Ser729) Rabbit Polyclonal Antibody (orb158163)
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