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Phospho-PPAR alpha (Ser12) Rabbit Polyclonal Antibody
SKU: orb6776
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Description
Research Area
Cell Biology, DNA and RNA, DNA and RNA Research, Epigenetics, Fatty Acid Oxidation, Fatty Acids, Hypoxia, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism, Mitochondrial Biogenesis, Nuclear hormone receptors (NHRs), Obesity, Zinc Finger Protein, mitochondrial transcription
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human, Mouse, Rabbit, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Porcine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12 PL(p-S)PL |
| Target | Ppara |
| Molecular Weight | 52 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−PPAR alpha(phospho-Ser12); PPAR alpha(phospho-S12); p-PPAR alpha(Ser12); p-PPAR alpha(S12); hPPAR; MGC2237; MGC2452; NR1C1; Nuclear receptor subfamily 1 group C member 1; Peroxisome Proliferator Activated Receptor alpha; PPAR; PPARA; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; PPARA_HUMAN; PPARalpha.
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phosphopeptide, non phosphopeptide.
Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Kidney (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Rat) Lysate at 40 ug, Lane 6: Jurkat (Human) Cell Lysate at 30 ug, Lane 7: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-PPAR alpha (Ser12) (orb6776) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 50 kD.
Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-PPAR alpha (Ser12) Polyclonal Antibody, Unconjugated (orb6776) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
Ppara
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Phospho-PPAR alpha (Ser12) Rabbit Polyclonal Antibody (orb6776)
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