Phospho-Smad2/Smad3 (Thr8) Rabbit Polyclonal Antibody

SKU: orb186235

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Description

Research Area

Cell Biology, Cell Signaling, Cytoplasmic, Intracellular Signaling Proteins

Images & Validation

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Item 1 of 5

Tested Applications	FC, WB
Dilution range	WB=1:500-2000, Flow-Cyt=1μg/Test
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Porcine, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8 PF(p-T)PP
Target	SMAD2
Molecular Weight	52 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Smad2+Smad3(phospho T8); p-Smad2+Smad3(phospho T8); P-Smad2/3(phospho T8); hMAD 2; hMAD 3; hSMAD2; hSMAD3; Mad related protein 2; MADH2; MADH3; MADR2; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma and Mad related protein 2; SMA and MAD related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3.

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Phospho-Smad2/Smad3 (Thr8) Rabbit Polyclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-Smad2/Smad3 (Thr8) antibody (orb186235), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

blank: A549 cells (blue line), isotype control: rabbit IgG (orange line), second antibody: goat anti-rabbit IgG (white blue line), primary antibody: rabbit Anti-phospho-Smad2/Smad3 (Thr8) (green line), contration: 3 µg/10^6 cells.

Sample: cerebrum (mouse) Lysate at 40 ug, RSC96 cell (rat) Lysate at 30 ug, hepG2 cell (human) Lysate at 30 ug, Hcclm3 cell (human) Lysate at 30 ug, Primary: Anti-p-Smad2/Smad3 (Thr8) (orb186235) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52kD, Observed band size: 48, 52 kD.

Sample: HUVEC cell (human) Lysate at 30 ug, Primary: Anti-p-Smad2/Smad3 (Thr8) (orb186235) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52kD, Observed band size: 52 kD.

Sample: U251 cell (human) Lysate at 30 ug, Primary: Anti-p-Smad2/Smad3 (Thr8) (orb186235) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52kD, Observed band size: 52 kD.

Quick Database Links

Gene Symbol

SMAD2

UniProt

Q15796

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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