Phospho-Vimentin (Ser56) Rabbit Polyclonal Antibody

SKU: orb5621

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Description

Images & Validation

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Tested Applications	FC, WB
Dilution range	WB=1:500-2000, Flow-Cyt=0.2μg /Test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Guinea pig, Porcine, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56 S(p-S)PG
Target	VIM
Molecular Weight	51 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Vimentin(phospho S56); p-Vimentin(phospho S56); FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.

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Phospho-Vimentin (Ser56) Rabbit Polyclonal Antibody

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-Phospho-Vimentin (Ser56) antibody (orb5621), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Lane 3: Hela (Human) Cell Lysate at 30 ug, Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 5: Spleen (Mouse) Lysate at 40 ug, Lane 6: Lymph node (Mouse) Lysate at 40 ug, Lane 7: Testis (Mouse) Lysate at 40 ug, Lane 8: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 9: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Vimentin (Ser56) (orb5621) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Quick Database Links

Gene Symbol

VIM

UniProt

P08670

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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