PPAR gamma Rabbit Polyclonal Antibody (FITC)

SKU: orb16249

Description

Research Area

Adipogenesis, Cardiovascular Research, Cell Biology, DNA and RNA Research, Diabetes Research, Fatty Acid Oxidation, Fatty Acids, Metabolism, Obesity, Zinc Finger Protein, cell Biology

Images & Validation

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Item 1 of 2

Tested Applications	FC, ICC, IF
Dilution range	Flow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Gallus, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human PPAR Gamma (101-200/505aa)
Target	PPARG
Purification	Affinity purified by Protein A
Conjugation	FITC

Storage & Handling

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Storage	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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GLM1; CIMT1; NR1C3; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; PPARG_HUMAN; PPAR-γ; PPAR γ; PPARγ;

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PPAR gamma Rabbit Polyclonal Antibody (FITC)

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-PPAR gamma antibody (orb16249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U251. Primary Antibody (green line): Rabbit Anti-NR1C3 antibody (orb16249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

PPARG

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Protocol Information

Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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