PPAR Gamma Rabbit Polyclonal Antibody

SKU: orb2101

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Description

Research Area

Adipogenesis, Cardiovascular Research, Cell Biology, DNA and RNA Research, Diabetes Research, Fatty Acid Oxidation, Fatty Acids, Metabolism, Obesity, Zinc Finger Protein, cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	FC, ICC, WB
Dilution range	WB=1:500-2000, ICC/IF=1:100-500, Flow-Cyt=1μg/Test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Gallus, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human PPAR Gamma (315-420/475aa)
Target	PPARG
Molecular Weight	57 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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GLM1; CIMT1; NR1C3; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; PPARG_HUMAN; PPAR-γ; PPAR γ; PPARγ;

Phospho-PPAR Gamma (Ser112) Rabbit Polyclonal Antibody [orb99282]

Applications:	ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Canine, Guinea pig, Porcine, Rabbit
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 100 μl, 50 μl, 200 μl

Item 1 of 5
Phospho-PPAR Gamma (ser273) Rabbit Polyclonal Antibody [orb158188]
Applications:
ELISA, WB
Predicted Reactivity:
Bovine, Gallus, Mouse, Rabbit, Rat, Sheep
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl
Item 1 of 7
Angiopoietin-like 4 Rabbit Polyclonal Antibody [orb101848]
Applications:
IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:
Bovine, Canine, Porcine, Rabbit, Sheep
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl
Item 1 of 5
PGC1 alpha + beta Rabbit Polyclonal Antibody [orb100963]
Applications:
FC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:
Bovine, Canine, Equine, Mouse, Porcine, Rabbit
Reactivity:
Human, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl, 200 μl

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Blank control (blue line): U251 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-PPAR Gamma antibody (orb2101), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: Liver (Mouse) Lysate at 40 ug, Heart (Mouse) Lysate at 40 ug, Lung (Mouse) Lysate at 40 ug, Primary: Anti-PPAR gamma (orb2101) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 51 kD.

Tissue/Cell: rat aorta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PPAR Gamma Polyclonal Antibody, Unconjugated (orb2101) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PPARG

UniProt

P37231

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ICC

Immunocytochemistry

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