PRDM13 Rabbit Polyclonal Antibody

Rating: 4
Author: Fadi Shaya

SKU: orb312614

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Description

PRDM13 Rabbit Polyclonal Antibody

Research Area

DNA and RNA Research, Zinc Finger Protein

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Hamster, Mouse
Predicted Reactivity	Bovine, Canine, Human, Porcine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human PRDM13 (551-650/707aa)
Target	PRDM13
Molecular Weight	74 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MU MB 20.220; PFM 10; PFM10; PR domain containing 13; PR domain containing protein 13; PR domain zinc finger protein 13; PRDM 13.

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Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PRDM13) Polyclonal Antibody, Unconjugated (orb312614) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: CHO (Hamster) Cell Lysate at 30 ug, Primary: Anti-PRDM13 (orb312614) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.

Quick Database Links

Gene Symbol

PRDM13

UniProt

Q9H4Q3

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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