Human Anti-Thyroid-Peroxidase Antibody (TPO-Ab) ELISA Kit

SKU: orb782852

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Description

The Human Anti-Thyroid-Peroxidase Antibody (TPO-Ab) ELISA Kit uses a competitive inhibition enzyme immunoassay technique to quantify Anti-Thyroid-Peroxidase Antibody (TPO-Ab) in human serum, plasma, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 3.13-200 ng/mL and a sensitivity of 1.14 ng/mL.

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Signal Transduction

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ng/mL
Sensitivity	1.14 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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TPO Ab ELISA Kit, TPO-Ab ELISA Kit

Quality Guarantee

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Reactivity:	Human
Dynamic Range:	3.125-200ng/ml
Sensitivity:	1.875ng/ml

Reactivity:	Human
Dynamic Range:	1.56-50ng/mL
Sensitivity:	1.56ng/mL

Reactivity:	Human
Dynamic Range:	0.312-20 ng/mL
Sensitivity:	0.112 ng/mL

Human Anti-Thyroid-Peroxidase Antibody (TPO-Ab) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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Protocol Information

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