Human Epidermal Growth Factor Receptor (EGFR) EasyStep ELISA Kit

SKU: orb2302962

Description

The Human Epidermal Growth Factor Receptor (EGFR) EasyStep ELISA Kit uses a sandwich enzyme immunoassay to quantify Epidermal Growth Factor Receptor (EGFR) EasyStep in human serum, plasma. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 25 pg/mL.

Research Area

Stem Cell & Developmental Biology; Cell Biology

Images & Validation

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Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR, and the Human EGFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR. After TMB substrate solution is added, only those wells that contain Human EGFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma
Assay Type	Sandwich
Assay Time	1.5h
Range	0.16-10 ng/mL
Sensitivity	25 pg/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Human Epidermal Growth Factor Receptor (EGFR) EasyStep ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

Quality Guarantee

Protocol Information

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