Human High Sensitivity C Reactive Protein (hs-CRP) ELISA Kit

SKU: orb782328

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Description

The Human High Sensitivity C Reactive Protein (hs-CRP) ELISA Kit uses a sandwich enzyme immunoassay to quantify High Sensitivity C Reactive Protein (hs-CRP) in human serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids. The kit has a detection range of 1.57-100 ng/mL and a sensitivity of 0.5 ng/mL.

Research Area

Cancer Biology, Cardiovascular Research, Infectious Disease & Virology, Musculoskeletal & Connective Tissue Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.5 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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C-RP ELISA Kit, PTX1 ELISA Kit, Pentraxin-Related ELISA Kit, Pentraxin 1 ELISA Kit, hs-CRP ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Find More Protocols

Reactivity:	Human
Dynamic Range:	15.63-1000pg/mL
Sensitivity:	5.47 pg/mL

Reactivity:	Human
Dynamic Range:	78.13-5000 pg/mL
Sensitivity:	21 pg/mL

Reactivity:	Human
Dynamic Range:	15.63-1000pg/mL
Sensitivity:	7.23pg/mL

Reactivity:	Human
Dynamic Range:	15.63-1000pg/mL
Sensitivity:	8.33pg/mL

Reactivity:	Human
Dynamic Range:	0.78 ng/mL-50ng/mL
Sensitivity:	0.39 ng/mL

Human High Sensitivity C Reactive Protein (hs-CRP) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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Protocol Information

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