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Human Insulin Like Growth Factor 1 Receptor (IGF1R) ELISA Kit

SKU: orb777260
Free Kit Promotion

Description

The Human Insulin Like Growth Factor 1 Receptor (IGF1R) ELISA Kit uses a sandwich enzyme immunoassay to quantify Insulin Like Growth Factor 1 Receptor (IGF1R) in human tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.126 ng/mL.

Research Area

Immunology & Inflammation; Metabolism Research; Stem Cell & Developmental Biology

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGF1R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGF1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGF1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGF1R in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample Typestissue homogenates, cell lysates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.126 ng/mL
Concentration20 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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CD221 ELISA Kit, IGF1-R ELISA Kit, IGFIR ELISA Kit, JTK13 ELISA Kit

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

UniProt Details

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No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
DispatchUsually dispatched within 5-10 working days
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