Human Nephrin (NPHN) ELISA Kit

SKU: orb775907

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Description

The Human Nephrin (NPHN) ELISA Kit uses a sandwich enzyme immunoassay to quantify Nephrin (NPHN) in human urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.056 ng/mL.

Research Area

Stem Cell & Developmental Biology; Kidney & Urological Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPHN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPHN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPHN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPHN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.056 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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NPHS1 ELISA Kit, CNF ELISA Kit, Nephrosis 1 ELISA Kit, Congenital Finnish Type ELISA Kit, Renal glomerulus-specific cell adhesion receptor ELISA Kit

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Quick Database Links

UniProt

O60500

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Human (1)

C Li, JKC Ng, GCK Chan, WWS Fung, KM Chow, CC Szeto Preservation of Urinary Podocyte Markers in Diabetic Kidney Disease by SGLT2 Inhibitor Therapy Kidney Diseases,, (2025)

PubMed:

Applications

ELISA

Reactivity

Human

Reactivity:	Human
Dynamic Range:	0.16-10ng/mL
Sensitivity:	0.10 ng/mL

Reactivity:	Human
Dynamic Range:	3.12-200pg/ml
Sensitivity:	1.875pg/ml

Reactivity:	Human
Dynamic Range:	156 pg/ml - 10000 pg/ml
Sensitivity:	80 pg/ml

Reactivity:	Human
Dynamic Range:	7.813-500ng/ml
Sensitivity:	4.688ng/ml

Reactivity:	Human
Dynamic Range:	0.16-10ng/mL
Sensitivity:	0.05ng/mL

Human Nephrin (NPHN) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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