Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) EasyStep ELISA Kit

SKU: orb2302948

Description

The Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) EasyStep ELISA Kit uses a sandwich enzyme immunoassay to quantify Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) EasyStep in human serum, plasma. The kit has a detection range of 93.75-6000 pg/mL and a sensitivity of 37.66 pg/mL.

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes

standard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma
Assay Type	Sandwich
Assay Time	1.5h
Range	93.75-6000 pg/mL
Sensitivity	37.66 pg/mL
Concentration	6000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha

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Human Soluble Interleukin-2 Receptor Alpha Chain (IL-2sRa/CD25) EasyStep ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

Quality Guarantee

Protocol Information

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