Human Stem Cell Factor Receptor (SCFR) ELISA Kit

SKU: orb775047

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Description

The Human Stem Cell Factor Receptor (SCFR) ELISA Kit uses a sandwich enzyme immunoassay to quantify Stem Cell Factor Receptor (SCFR) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 1.57-100 ng/mL and a sensitivity of 0.62 ng/mL.

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCFR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.62 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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CD117 ELISA Kit, KIT ELISA Kit, C-Kit ELISA Kit, PBT ELISA Kit, p145 c-Kit ELISA Kit, C-Kit Receptor ELISA Kit, Tyrosine-Protein Kinase ELISA Kit, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog ELISA Kit, Piebald Trait ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

UniProt

P10721

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Reactivity:	Human
Dynamic Range:	0.79-50ng/mL
Sensitivity:	0.47 ng/mL

Applications:	ELISA, ICC, IF, IHC-P
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, WB
Reactivity:	Human, Mouse, Rat
Clonality:	Polyclonal
Conjugation:	Unconjugated

Reactivity:	Human
Dynamic Range:	1.57-100 ng/mL
Sensitivity:	0.6 ng/mL

Applications:	ELISA, FC, IHC
Reactivity:	Human
Clonality:	Monoclonal
Conjugation:	Unconjugated

Human Stem Cell Factor Receptor (SCFR) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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UniProt Details

Protocol Information

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