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Human Vascular Endothelial Cadherin (VE-Cadherin) EasyStep ELISA Kit

SKU: orb2302947
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Description

The Human Vascular Endothelial Cadherin (VE-Cadherin) EasyStep ELISA Kit uses a sandwich enzyme immunoassay to quantify Vascular Endothelial Cadherin (VE-Cadherin) EasyStep in human serum, plasma. The kit has a detection range of 0.16-10 µg/mL and a sensitivity of 32.1 ng/mL.

Research Area

Cardiovascular Research

Images & Validation

Application Notes
standard: 10 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin, and the Human VE-Cadherin standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VE-Cadherin. After TMB substrate solution is added, only those wells that contain Human VE-Cadherin and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityHuman
Sample TypesSerum, plasma
Assay TypeSandwich
Assay Time1.5h
Range0.16-10 µg/mL
Sensitivity32.1 ng/mL
Concentration10 μg/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5
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Protocol Information

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48 T
$ 330.00
96 T
$ 470.00
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