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Featured
Description
Research Area
Epigenetics & Chromatin
Images & Validation
−
Item 1 of 7
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; IF/ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | B3C33H0 |
| Immunogen | Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli. |
| Target | MGMT |
| Molecular Weight | 21.6kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EC 2.1.1.63
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
Rodriguez, Fausto J. et al. MGMT immunohistochemical expression and promoter methylation in human glioblastoma Appl Immunohistochem Mol Morphol, 16, 59-65 (2008)
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