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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−
Item 1 of 4
| Tested Applications | FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine, Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Histone H2AX around the phosphorylation site of Tyr139 QA(p-S)QE |
| Target | H2AX |
| Molecular Weight | 15 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H2A.X; H2A/X; H2AFX; Hist5-2ax; gammaH2ax; RGD1566119; H2AX_HUMAN; H2AX; Histone H2A.X; H2AX_MOUSE; H2A.X variant histone; H2A histone family, member X; H2A histone family member X; H2AX (phospho-S139); H2AXS139P; p-H2AX; phospho-H2AX; γH2AX; H2AX (phospho-Ser139)
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Quick Database Links
Gene Symbol
H2AX
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
Available Sizes
Select a size below
Free Secondary Antibody (20 ul)0/0
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