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Rat Claudin 18 (CLDN18) ELISA Kit

SKU: orb780431
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Description

The Rat Claudin 18 (CLDN18) ELISA Kit uses a sandwich enzyme immunoassay to quantify Claudin 18 (CLDN18) in rat tissue homogenates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.061 ng/mL.

Research Area

Cancer Biology

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CLDN18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CLDN18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CLDN18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CLDN18 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityRat
Sample TypesTissue homogenates and other biological fluids.
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.061 ng/mL
Concentration10 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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SFTP-J ELISA Kit, SFTPJ ELISA Kit, SP-J ELISA Kit, SPJ ELISA Kit, Surfactant Protein J ELISA Kit, Surfactant Associated Protein J ELISA Kit

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Quality Guarantee

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
DispatchUsually dispatched within 5-10 working days
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