PSAP Rabbit Polyclonal Antibody

SKU: orb13663

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Description

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Canine, Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Prosaposin (101-200/524aa)
Target	PSAP
Molecular Weight	8.8/58 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Prosaposin; A1 activator; Cerebroside sulfate activator; Co-beta-glucosidase; Component C; CSAct; Dispersin; GLBA; Glucosylceramidase activator; Proactivator polypeptide; Proactivator polypeptide precursor; Prosaposin(sphingolipid activator protein 1); prosaposin(variant Gaucher disease and variant metachromatic leukodystrophy); Protein A; Protein C; PSAP; SAP-1; SAP-2; SAP_HUMAN; SAP1; Saposin A; Saposin B; Saposin B Val; Saposin C; Saposin D; Saposin-D; Saposins; Sgp1; Sphingolipid activator protein 1; Sphingolipid activator protein 2; Sulfated glycoprotein 1; Sulfatide/GM1 activator.

SFTPA2 Rabbit Polyclonal Antibody [orb326486]

Applications:	WB
Predicted Reactivity:	Bovine, Canine, Equine, Goat, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 100 μl

Item 1 of 2
PSAP Antibody [orb630268]
Applications:
ELISA, IF, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg, 50 μg

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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PSAP) Polyclonal Antibody, Unconjugated (orb13663) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Protein: testis (rat) lysates at 30 ug, brain (rat) lysates at 30 ug, Primary: Anti-Anti-PSAP/PAP (orb13663) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, ECL excitated the fluorescence, Predicted band size: 58kD, Observed band size: 58kD.

Sample: Testis (Rat) Lysate at 40 ug, BV-2 (Mouse) CellLysate at 30 ug, Primary: Anti-PSAP (orb13663) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 8.8/58 kD, Observed band size: 58 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PSAP/PAP Polyclonal Antibody, Unconjugated (orb13663) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PSAP

UniProt

P07602

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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