RAMP1 Rabbit Polyclonal Antibody

SKU: orb10480

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Human, Rat
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human RAMP1 (118-148/148aa)
Target	RAMP1
Molecular Weight	14 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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calcitonin receptor like receptor activity modifying protein 1; RAMP 1; RAMP-1; CRLR activity modifying protein 1; receptor(G protein coupled) activity modifying protein 1; receptor activity modifying protein 1; Receptor activity modifying protein 1; RAMP1_HUMAN.

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Sample: Lane 1: Recombinant human RAMP1 protein, Primary: Anti-RAMP1 (orb10480) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: / kDa, Observed band size: 32 kDa.

Formaldehyde fixed and paraffin embedded rat kidneys labeled with Rabbit Anti-RAMP1 Polyclonal Antibody (orb10480) and followed by conjugation to a secondary antibody and counterstained with hematoxylin.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-RAMP1 Polyclonal Antibody, Unconjugated (orb10480) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

RAMP1

UniProt

O60894

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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