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ROR gamma T Rabbit Polyclonal Antibody
SKU: orb500718
Featured
Description
Research Area
Corticoid, DNA and RNA Research, Hormone Research, Inflammation, Transcription Factors
Images & Validation
−Item 1 of 4
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse RORC (1-100/516aa) |
| Target | RORC |
| Molecular Weight | 54 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−RORG_HUMAN; Nuclear receptor ROR-gamma; ROR gamma; NR1F3; RZRG; TOR; IMD42; RZR-GAMMA; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; ROR gamma T [Mus musculus]; nuclear receptor ROR-gamma isoform 2; RORgT;
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Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ROR gamma T) Polyclonal Antibody, Unconjugated (orb500718) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ROR gamma T) Polyclonal Antibody, Unconjugated (orb500718) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Muscle Lysates, Lane 2: Rat Muscle Lysates, Primary: Anti-ROR Gamma (orb500718) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 50 kD.
Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-ROR gamma T (orb500718) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Quick Database Links
Gene Symbol
RORC
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
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Filter by Species
Shiyou Wei # 1 2, Dandan Ling # 3 2, Jingui Zhong 4, Rui Chang 5, Xinyu Ling 6, Zhigang Chen 1, Ruowang Duan Elk1 enhances inflammatory cell infiltration and exacerbates acute lung injury/acute respiratory distress syndrome by suppressing Fcgr2b transcription Mol Med, 30, 53 (2024)
Applications
IHC
Reactivity
Rat
ROR gamma T Rabbit Polyclonal Antibody (orb500718)
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