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Runx3 Rabbit Polyclonal Antibody

SKU: orb11342

Description

Runx3 Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P, WB
Dilution RangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman, Mouse
Predicted ReactivityBovine, Canine, Gallus, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Runx3 (151-250/415aa)
TargetRUNX3
Molecular Weight44 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

RUNX3_HUMAN; Runt-related transcription factor 3; AML2; CBFA3; PEBP2A3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3(CBF-alpha-3); Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit(PEA2-alpha C; PEBP2-alpha C); SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit;

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Runx3 Rabbit Polyclonal Antibody

Sample: A549 Cell Lysate at 30 ug, Primary: Anti-Runx3 (orb11342) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 44 kD.

Runx3 Rabbit Polyclonal Antibody

Sample: Lymph node (Mouse) Lysate at 30 ug, Primary: Anti-Runx3 (orb11342) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 44 kD.

Runx3 Rabbit Polyclonal Antibody

Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Runx3 Polyclonal Antibody, Unconjugated (orb11342) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Runx3 Rabbit Polyclonal Antibody (orb11342)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
DispatchUsually dispatched within 5-10 working days
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