Sertad1 Rabbit Polyclonal Antibody

SKU: orb100366

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Description

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse
Predicted Reactivity	Bovine, Canine, Human, Porcine, Rabbit, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human SEI1 (1-42/236aa)
Target	SERTAD1
Molecular Weight	25 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CDK 4 binding protein p34SEI1; CDK4 binding protein p34SEI; CDK4 binding protein p34SEI1; SEI 1; SEI1; SERTA domain containing 1; SERTA domain containing 1 variant; SERTA domain containing protein 1; Sertad 1; Transcriptional regulator interacting with the PHD bromodomain 1; TRIP Br1; SRTD1_HUMAN.

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Tissue/cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Sertad1 Polyclonal Antibody, Unconjugated (orb100366) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: Mouse embryos tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Sertad1 Polyclonal Antibody, Unconjugated (orb100366) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SERTAD1

UniProt

Q9UHV2

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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IHC (2)

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Human (2)

Jung, Samil et al. Oncogenic function of p34SEI-1 via NEDD4‑1‑mediated PTEN ubiquitination/degradation and activation of the PI3K/AKT pathway Int J Oncol, 43, 1587-1595 (2013)

PubMed: 23970032

Applications

IHC

Reactivity

Human

Wu, Min-Ju et al. KLF10 affects pancreatic function via the SEI-1/p21Cip1 pathway Int J Biochem Cell Biol, 60, 53-59 (2015)

PubMed: 25578559

Applications

IHC

Reactivity

Human