SHH Rabbit Polyclonal Antibody

SKU: orb579101

Description

Rabbit polyclonal antibody to SHH

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Molecular Biology, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 7

Tested Applications	IHC, WB
Reactivity	Gallus, Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Goat, Guinea pig, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human SHH
Target	SHH
Molecular Weight	28 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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TPT, HHG1, HLP3, HPE3, SMMCI, ShhNC, TPTPS, MCOPCB5

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Chicken embryos, Primary antibody: 1:1000 (orb579101) anti-shh Secondary Antibody: 1:500 (ASP00001) goat anti-rabbit HRP conjugated.

Sample Tissue: Mouse Brain, Antibody Dilution: 1 ug/ml.

Sample Tissue: Human Stomach Tumor, Antibody Dilution: 1.0 ug/ml.

Rabbit Anti-SHH Antibody, Catalog Number: orb579101, Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue, Observed Staining: Plasma membrane, Cytoplasm, Primary Antibody Concentration: 1:100, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

Sample Type: Human glioma cells, Primary Antibody Dilution: 1:200, Secondary Antibody: Anti-rabbit-GFP, Secondary Antibody Dilution: 1:500, Color/Signal Descriptions: 1. SHH: Green 2. DAPI: Blue 3. Merge, Gene Name: SHH.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.

Quick Database Links

Gene Symbol

SHH

UniProt

Q15465

RefSeq (Protein):NP_000184

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000184

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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