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STAT3 Mouse Monoclonal Antibody
SKU: orb500764
Featured
Description
Images & Validation
−Item 1 of 9
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:1000-5000, IHC-P=1:200-2000, IHC-F=1:200-2000, IF=1:200-2000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 3F5 |
| Immunogen | Recombinant human STAT3 Protein (9-265/770aa) |
| Target | STAT3 |
| Molecular Weight | 88 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−1110034C02Rik; Acute Phase Response Factor; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC128731; MGC16063; MGC93551; Signal transducer and activator of transcription 3(acute-phase response factor); Signal transducer and activator of transcription 3; Signal Transductor and Activator of Transcription 3; STAT 3; Transcription factor; STAT3_HUMAN.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (ascites of orb500764) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (orb500764) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Polyclonal Antibody, Unconjugated (orb500764) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (orb500764) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Polyclonal Antibody, Unconjugated (orb500764) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (ascites of orb500764 3F5) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Polyclonal Antibody, Unconjugated (orb500764) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Mouse Raw264.7 cell lysates, Lane 3: Human HepG2 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human SW480 cell lysates, Lane 6: Human THP-1 cell lysates, Primary: Anti-STAT3 (orb500764) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 88 kDa, Observed band size: 88 kDa.
Sample: Lane 1: STAT3 knockout (KO) A549 Cell Lysate, Lane 2: Human A549 Cell (Control) Lysate, Primary: Anti-STAT3 (orb500764) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 88 kD, Observed band size: 88 kD.
Quick Database Links
Gene Symbol
STAT3
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
STAT3 Mouse Monoclonal Antibody (orb500764)
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