STAT3 Rabbit Polyclonal Antibody

SKU: orb499707

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Description

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human STAT3 (151-250/770aa)
Target	STAT3
Molecular Weight	88 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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1110034C02Rik; Acute Phase Response Factor; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC128731; MGC16063; MGC93551; Signal transducer and activator of transcription 3(acute-phase response factor); Signal transducer and activator of transcription 3; Signal Transductor and Activator of Transcription 3; STAT 3; Transcription factor; STAT3_HUMAN.

Phospho-STAT3 (Tyr705) Rabbit Polyclonal Antibody [orb608174]

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Bovine, Canine, Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 100 μl, 200 μl, 50 μl

Item 1 of 6
STAT3 Rabbit Polyclonal Antibody [orb592766]
Applications:
IHC, WB
Predicted Reactivity:
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl
Item 1 of 7
Stat3 Polyclonal Antibody [orb1412088]
Applications:
IF, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl

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Paraformaldehyde-fixed, paraffin embedded (Human breast cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Polyclonal Antibody, Unconjugated (orb499707) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: RSC96 cells (pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C), Primary Antibody: Rabbit Anti-STAT3 antibody (orb499707) (green), Concentration: 0.2 µg/10^6 cells, Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: F (ab')2 fragment goat anti-rabbit IgG-PE (white blue).

Quick Database Links

Gene Symbol

STAT3

UniProt

P40763

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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