TIGIT Rabbit Polyclonal Antibody

SKU: orb1499421

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Description

Research Area

Immunology, SARS /COVID-19, Stem Cell Research

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution Range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Human

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant human TIGIT protein (22-141/244aa)
Target	TIGIT
Molecular Weight	26 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; V-set and immunoglobulin domain-containing protein 9; V-set and immunoglobulin domain-containing protein 3; TIGIT_HUMAN;

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Measured by its binding ability in a indirect ELISA. Immobilized Human TIGIT protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human TIGIT Antibody, the EC50 is 1070 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human TIGIT protein, HisTag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human TIGIT Antibody, the EC50 is 152.5 ng/ml.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Rat Lymph node tissue lysates, Primary: Anti-TIGIT (orb1499421) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26 kDa, Observed band size: 35 kDa.

Quick Database Links

Gene Symbol

TIGIT

UniProt

Q495A1

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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