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Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−
Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human TLR2 (1-600/784aa) |
| Target | TLR2 |
| Molecular Weight | 100 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD282; TIL4; TLR2_HUMAN; TLR2; Toll/interleukin-1 receptor-like protein 4; toll like receptor 2; toll-like receptor 2
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Quick Database Links
Gene Symbol
TLR2
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
Filter by Applications
Filter by Species
Ezz, Mohamed Aboul et al. TLR2/4 signaling pathway mediates sperm-induced inflammation in bovine endometrial epithelial cells in vitro PLoS ONE, 14, e0214516 (2019)
Applications
IHC
Reactivity
Bovine
Bishayi, B. et al. Possible role of Toll-like receptor-2 in the intracellular survival of Staphylococcus aureus in murine peritoneal macrophages: involvement of cytokines and anti-oxidant enzymes Scand J Immunol, 80, 127-143 (2014)
Applications
WB
Reactivity
Mouse
Kowsar, Rasoul et al. Regulation of innate immune function in bovine oviduct epithelial cells in culture: the homeostatic role of epithelial cells in balancing Th1/Th2 response J Reprod Dev, 59, 470-478 (2013)
Applications
IHC
Reactivity
Bovine
Rituparna Ghosh 1, Biswadev Bishayi Neutralization of TLR2 in combination with either TNF-α or IL-1β antibody reduces the severity of septic arthritis through STAT3/mTOR signalling in lymphocytes Cell Immunol ., (2024)
Rana Adhikary, Sahin Sultana, Biswadev Bishayi Clitoria ternatea flower petals: Effect on TNFR1 neutralization via downregulation of synovial matrix metalloproteases Journal of Ethnopharmacology, 210, 209 (2017)
Applications
WB
Reactivity
Mouse
I Akthar, Y Kim, T Umehara, C Kanno Activation of sperm Toll-like receptor 2 induces hyperactivation to enhance the penetration to mucus and uterine glands: a trigger for the uterine inflammatory cascade Frontiers in Immunology, (2024)
Applications
IF
Ajeya Nandi, Biswadev Bishayi A novel CCR-2/TLR-2 triggered signaling in murine peritoneal macrophages intensifies bacterial (Staphylococcus aureus) killing by reactive oxygen species through TNF-R1 Immunology Letters, 190, (2017)
Applications
Blocking, WB
Reactivity
Human
Rituparna Ghosh 1, Biswadev Bishayi Endogenous blocking of TLR2 along with TNF-α and IL-1β ameliorates the severity of the S. aureus arthritis via modulating STAT3/SOCS3 expressions in tissue resident macrophages Microb Pathog, (2024)
Malinda Hulugalla 1, Alireza Mansouri 1, Elham Waehama 1 2, Ihshan Akthar 1, Akio Miyamoto Semen extender triggers a mild physiological inflammatory response in the uterus without disrupting sperm-uterine immune crosstalk in vitro in cattle J Reprod Dev, (2024)
Dongxue Ma 1, Ihshan Akthar 1, Taiyo Hashimoto 1, Mohamed A Marey 1 2, Kazuya Kusama 3, Kazuhiko Imakawa 4, Masayuki Shimada 5, Akio Miyamoto Toll-like receptor 2 activation of early divided bovine embryo promotes its viability and development competence in vitro Sci Rep, (2025)
Applications
IF, WB
Reactivity
Bovine
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Mol Oral Microbiol, 38, 158-170 (2023)
Elham WAEHAMA A diversity of uterine immune response to peptidoglycans (PGNs) derived from different bacterial species in cattle Obihiro University of Agriculture and Veterinary Medicine, (2025)
Applications
IF
Reactivity
Bovine
Dey, Rajen et al. Ascorbic acid along with ciprofloxacin regulates S. aureus induced microglial inflammatory responses and oxidative stress through TLR-2 and glucocorticoid receptor modulation Inflammopharmacology, 30, 1303-1322 (2022)
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