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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Item 1 of 5
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from rat TLR4 (751-835/835aa) |
| Target | TLR4 |
| Molecular Weight | 120 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ARMD10; CD284; TLR-4; TOLL; Lps; Ly87; Ran/M1; Rasl2-8; TLR4_HUMAN; TLR4; hToll; TLR4_MOUSE; TLR4_RAT; Toll4; toll like receptor 4; toll-like receptor 4
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Quick Database Links
Gene Symbol
TLR4
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Filter by Applications
Filter by Species
Colak, Ozlem et al. Evaluation of Safe Systemic Immunosuppression Created with Dexamethasone in Prevention of Capsular Contracture: A Glance to Distinct Perspectives with Toll-Like Receptors Aesthetic Plast Surg, 42, 1133-1143 (2018)
Applications
IHC
Reactivity
Rat
Leopoldo Cosme-Silva a 1 , Renan Dal-Fabbro b 1 , Fernanda de Lima Pontes a , Leticia Cabrera Capalbo c , Edilson Ervolino d , Luciano Tavares Angelo Cintra d , Juan José Segura-Egea e , João Eduardo Gomes Filho Multi-Strain Probiotic Formula Modulates Expression of β-Defensin-2, β-Defensin-3, and TLR-4 in Male Rats with Apical Periodontitis Archives of Oral Biology, (2024)
Applications
IHC
Reactivity
Rat
David Zapico 1 2, José Espinosa 1 2, Miguel Criado 1 2, Daniel Gutiérrez 1 2, María Del Carmen Ferreras 1 2, Julio Benavides 2, Valentín Pérez 1 2, Miguel Fernández Immunohistochemical expression of TLR1, TLR2, TLR4, and TLR9 in the different types of lesions associated with bovine paratuberculosis Vet Pathol ., (2024)
Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Mol Oral Microbiol, 38, 158-170 (2023)
Applications
IHC
Reactivity
Rat
Ostadhadi, Sattar et al. Mesenteric artery responsiveness to acetylcholine and phenylephrine in cirrhotic rats challenged with endotoxin: the role of TLR4 Can J Physiol Pharmacol, 93, 475-483 (2015)
Applications
IHC
Reactivity
Rat
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