TNF alpha Mouse Monoclonal Antibody

SKU: orb500520

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Description

Research Area

Cancer Research, Growth Factors, Immunology, Inflammation, Inflammatory Mediators, Metabolism, SARS /COVID-19

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution Range	WB=1:1000-5000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Gallus, Guinea pig, Insect, Monkey, Mouse, Porcine, Rabbit, Sheep, Yeast

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	3A4
Immunogen	Recombinant human TNF alpha 77-233aa
Target	TNF
Molecular Weight	17/26 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor necrosis factor-α; TNFalpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF-alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor alpha.

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Sample: plasma (Rat) at 40 ug serum (Rat) at 40 ug Primary: Anti-TNF-alpha (orb500520) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17/26 kD Observed band size: 26 kD.

Quick Database Links

Gene Symbol

TNF

UniProt

P01375

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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WB (1)

Mingcai Duan 1, Jinyu Liu 2, Zhaoxia Cai 3, Li Chen 2, Yong Tian 2, Wenwu Xu 2, Tao Zeng 2, Tiantian Gu 4, Lizhi Lu Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks Ecotoxicol Environ Saf, (2025)

PubMed: 39914079

Applications