Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human TNF-alpha (181-235/235aa)
Target	TNF
Molecular Weight	17/26 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Tumor necrosis factor-α; TNFalpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF-alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor alpha.

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Sample: Diseased lung (mouse) at 30 ug, Primary: Anti-TNF alpha (orb7100) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000 dilution, Predicted band size: 17/28 kD, Observed band size: 17/28 kD.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-TNF alpha (orb7100) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36/17/26 kD, Observed band size: 36 kD.

Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-TNF alpha (orb7100) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/26kD, Observed band size: 25 kD.

Quick Database Links

Gene Symbol

TNF

UniProt

P01375

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ICC

Immunocytochemistry

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Filter by Applications

IHC (3)
WB (1)

Filter by Species

Mouse (1)
Rat (2)

Oh, Ha Na et al. Effects of Quercetin in a Mouse Model of Experimental Dry Eye Cornea, 34, 1130-1136 (2015)

PubMed: 26203745

Applications

IHC

Reactivity

Mouse

Correia, Marcelo Henrique et al. Immune response and Raman scattering assessment in rats skin after contact with Fusarium oxysporum metabolites Spectrochim Acta A Mol Biomol Spectrosc, 234, 118246 (2020)

PubMed: 32179464

Applications

IHC

Reactivity

Rat

Shi, Xiaowei et al. Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats Metab Brain Dis, 32, 221-233 (2017)

PubMed: 27585466

Applications

IHC, WB

Reactivity

Rat