Tubb3 Rabbit Polyclonal Antibody

SKU: orb500995

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Description

Research Area

Neurology, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Tubb3 (301-400/450aa)
Target	TUBB3
Molecular Weight	50 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Tubulin beta 3; beta III Tubulin; Tubb3; Tubulin beta-3; beta 4; MC1R; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Neuron-specific class III beta-tubulin; Syntaxin III; Neuron specific beta III Tubulin; Tubulin beta-4 chain; Tubulin beta-III; beta-4; CDCBM; CFEOM3A; M(beta)3; M(beta)6; Neuron-specific class III beta-tubulin; QccE-11995; QccE-15186; TBB3_HUMAN; Tubulin beta 4; Tubulin beta-4 chain.

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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tubb3) Polyclonal Antibody, Unconjugated (orb500995) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tubb3) Polyclonal Antibody, Unconjugated (orb500995) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) lysate at 30 ug, Primary: Anti-Tubb3 (orb500995) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Lane 1: Mouse Brain Lysates, Lane 2: Mouse Cerebellum Lysates, Lane 3: Rat Brain Lysates, Lane 4: Rat Cerebellum Lysates, Lane 5: Human SH-SY5Y cell Lysates, Primary: Anti-Tubb3 (orb500995) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

TUBB3

UniProt

Q13509

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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