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TWIST1 Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human TWIST1 |
| Target | TWIST1 |
| Molecular Weight | 21kDa |
| Purification | Protein A purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 1.0 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−TWIST Rabbit Polyclonal Antibody [orb13736]
IF, IHC-Fr, IHC-P
Human
Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 50 μl, 100 μlTWIST1 Rabbit Polyclonal Antibody [orb1562792]
ELISA, WB
Mouse, Rat
Human
Rabbit
Polyclonal
Unconjugated
100 μg

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Sample Type: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5 ug/mL, Peptide Concentration: 2.0 ug/mL, Lysate Quantity: 25 ug/lane, Gel Concentration: 12%.

Positive control (+): A549 (N03), Negative control (-): U937 (N31), Antibody concentration: 1 ug/mL.

Uterus

WB Suggested Anti-TWIST1 Antibody Titration: 2.5 ug/mL, ELISA Titer: 1:1562500, Positive Control: Jurkat cell lysate.
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Protocol Information
Armando, Federico et al. Epithelial to Mesenchymal Transition (EMT) in a Laryngeal Squamous Cell Carcinoma of a Horse: Future Perspectives Animals (Basel), 10, E2318 (2020)
TWIST1 Rabbit Polyclonal Antibody (orb329955)
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