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Tyrosine Hydroxylase Rabbit Polyclonal Antibody
SKU: orb11539
Featured
Description
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Bovine, Canine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse TH (101-165/528aa) |
| Target | TH |
| Molecular Weight | 60 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−DYT14; DYT5b; ple; Protein Pale; c; The; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyk2; TY3H_HUMAN; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.
Sample: adipocyte (mouse) Lysate at 5-10 ug, model A, model B, model C are from different mice, Primary: Anti-Tyrosine Hydroxylase (orb11539) at 1/2000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.
Sample: PC-3 (Mouse) Lysate at 30 ug, Primary: Anti-Tyrosine Hydroxylase (orb11539) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (orb11539) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
TH
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Tyrosine Hydroxylase Rabbit Polyclonal Antibody (orb11539)
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