VEGF Rabbit Polyclonal Antibody

SKU: orb704380

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Description

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant human VEGF165 Protein (27-191/191aa)
Target	VEGFA
Molecular Weight	22 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGFA; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF A Precursor.

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Sample: Lane 1: Huvec (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Lane 4: THP-1 (Human) Cell Lysate at 30 ug, Lane 5: A431 (Human) Cell Lysate at 30 ug, Lane 6: U87MG (Human) Cell Lysate at 30 ug, Lane 7: Hela (Human) Cell Lysate at 30 ug, Lane 8: Jurkat (Human) Cell Lysate at 30 ug, Lane 9: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-VEGF (orb704380) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 28 kD.

Quick Database Links

Gene Symbol

VEGFA

UniProt

P15692

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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