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VEGFA Rabbit Polyclonal Antibody

SKU: orb500651
FeaturedFeatured Product

Description

VEGFA Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman, Rat
Predicted ReactivityCanine

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human VEGFA (101-200/232aa)
TargetVEGFA
Molecular Weight24 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPreservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; VEGF-A; Vascular permeability factor(VPF);

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

VEGFA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (orb500651) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

VEGFA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (orb500651) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

VEGFA Rabbit Polyclonal Antibody

Sample: Cerebellum (Rat) Lysate at 40 ug, Kidney (Rat) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Liver (Rat) Lysate at 40 ug, Primary: Anti-VEGFA (orb500651) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43/23 kD, Observed band size: 23 kD.

VEGFA Rabbit Polyclonal Antibody

Sample: Lane 1: Human K562 cell lysates, Lane 2: Human MDA-MB-231 cell lysates, Primary: Anti-VEGFA (orb500651) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kDa, Observed band size: 24 kDa.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

VEGFA Rabbit Polyclonal Antibody (orb500651)

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Available Sizes

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
DispatchUsually dispatched within 5-10 working days
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