ZO-1/TJP1 Rabbit Polyclonal Antibody (APC)

SKU: orb1005914

Description

ZO-1/TJP1 Rabbit Polyclonal Antibody (APC) is a APC conjugated antibody targeting TJP1. This antibody is suitable for FC, ICC. It exhibits reactivity with Human samples.

Research Area

Signal Transduction

Images & Validation

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Tested Applications	FC, ICC
Dilution Range	Flow-Cyt=1μg/Test, ICC/IF=1:50-200
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Gallus, Goat, Guinea pig, Mouse, Porcine, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human ZO-1 (1551-1702/1733aa)
Target	TJP1
Purification	Affinity purified by Protein A
Conjugation	APC

Storage & Handling

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Storage	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ZO1_HUMAN; Tight junction protein ZO-1; TJP1; ZO1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; ZO1 tight junction protein;

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ZO-1/TJP1 Rabbit Polyclonal Antibody (APC)

Blank control: A431. Primary Antibody (green line): Rabbit Anti-ZO-1 antibody (orb1005914), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-ZO-1 antibody (orb1005914), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

TJP1

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Protocol Information

Flow Cytometry

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ICC

Immunocytochemistry

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