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14-3-3 β rabbit pAb Antibody
SKU: orb764415
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 beta. AA range:41-90 |
| Molecular Weight | 28 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-YWHAB antibody, anti-14-3-3 protein beta/alpha antibody, anti-Protein 1054 antibody, anti-Protein kinase C inhibitor protein 1 antibody, anti-KCIP-1 antibody
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Western Blot analysis of various cells using 14-3-3 β Polyclonal Antibody.
Western Blot analysis of Jurkat cells using 14-3-3 β Polyclonal Antibody.
Western blot analysis of lysates from HepG2 and Jurkat cells, using 14-3-3 beta Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HepG2 cells using 14-3-3 β antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
14-3-3 β rabbit pAb Antibody (orb764415)
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